- Catalog number
- ICH1163-100mg
- Size / count
- 100 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD25 (IL-2Rα) is the 55 kDa subunit of the interleukin 2 receptor alpha chain which has been shown to play roles in lymphocyte differentiation, activation, and proliferation. IL-2Rα is a transmembrane glycoprotein comprised of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. IL-2Rα heterodimerizes with IL-2Rβ (CD122) and IL-2Rγ (CD132) and forms the IL-2 receptor complex. IL-2Rα by itself binds IL-2 with relatively low affinity, while the IL-2 receptor complex binds IL-2 with high affinity. IL-2Rα mutations cause severe immunodeficiency.
- CAS Number
- 9007-83-4
- Specifications
- Clone 7G7B6 reacts with human CD25
- Product Name Alternative
- IL2RA, Interleukin-2 receptor subunit alpha, TAC, p55
- UniProt
- P01589
- Host
- Mouse
- Species Reactivity
- Human
- Target
- CD25
- Isotype
- IgG2a
- Clone
- 7G7B6
- Applications
- ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
- Purification Method
- This monoclonal antibody was purified using Protein A
- Concentration
- 1mg/mL
- Endotoxin
- ≤ 1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE and HPLC
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free
- Antigen Distribution
- IL-2Rα is expressed on activated mature T and B lymphocytes, during early stages of thymocytes development, pre-B cells, and in activated CD4+ memory T-lymphocytes
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Mouse-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-C1.18.4-ICH2245