- Catalog number
- ICH1172-50mg
- Size / count
- 50 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CCR5 antibody, HEK/1/85a/7a, recognizes C-C chemokine receptor type 5 (CCR5), also known as CD195, CC-CKR-5, CHEMR13, and HIV-1 fusion coreceptor. CCR5 is a 45 kDa seven-transmembrane G-protein-coupled receptor (GPCR). CCR5 is expressed on a subset of memory T cells, macrophages, and immature dendritic cells (DCs) and is upregulated by proinflammatory cytokines. CCR5 binds to inflammatory CC-chemokines, including CCL3 (macrophage inflammatory protein (MIP)-1α), CCL4 (MIP-1β), and RANTES, which increases intracellular calcium levels and results in cytoskeletal rearrangement and chemotactic cell migration. CCR5 is also the coreceptor for HIV-1 following binding of viral gp120 to CD44-7. The levels of CCR5 on cells influence the rate of entry of HIV-1, and patients with lower levels of CCR5 exhibit immunity against infection. CCR5 also contributes to other human diseases, including type I diabetes and cancer.
- CAS Number
- 9007-83-4
- Specifications
- Clone HEK/1/85a/7a recognizes an epitope on human CCR5
- Product Name Alternative
- C-C chemokine receptor type 5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor, CD195
- UniProt
- P51681
- Host
- Rat
- Species Reactivity
- Human
- Target
- CCR5
- Isotype
- IgG2b Kappa
- Clone
- HEK/1/85a/7a
- Applications
- Flow Cytometry, ImmunoCytochemistry
- Purification Method
- This monoclonal antibody was purified using Protein G
- Concentration
- ≥ 5.0 mg/mL
- Endotoxin
- <1.0 EU/mg as determined by the LAL method
- Purity
- ≥95% monomer by analytical SEC, >95% by SDS Page
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
- Antigen Distribution
- CCR5 is expressed on memory T cells, macrophages, and immature dendritic cells (DCs)
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- The suggested concentration for this CD195 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
- Isotype Control
- https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243
