Bulk Anti-Human CD5 (UCHT-2) Antibody

Catalog number ICH1174-50mg

Price for ICH1174-50mg

Size / count 50 mg

Specifications

Catalog number: ICH1174-50mg
Size / count: 50 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas.
CAS Number: 9007-83-4
Specifications: Mouse Anti-Human CD5 (Clone UCHT-2) recognizes Human CD5. Anti-Human CD5 recognizes a human T-lymphocyte surface membrane antigen (Mr 67 kDa).
Product Name Alternative: Leu-1
UniProt: P06127
Host: Mouse
Species Reactivity: Human
Target: CD5
Isotype: IgG1
Clone: UCHT-2
Applications: CODEX®, Functional Assays, Flow Cytometry, IHC (Frozen)
Purification Method: This monoclonal antibody was purified using Protein A
Concentration: ≥ 5.0 mg/mL
Endotoxin: <1.0 EU/mg as determined by the LAL method
Purity: ≥95% monomer by analytical SEC, >95% by SDS Page
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Antigen Distribution: The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and >90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.1 Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.
Aggregation: Aggregation level ≤ 5%
Applications Notes: The suggested concentration for this CD5 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Isotype Control: https://www.ichor.bio/product/Mouse-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-HKSP-ICH2247

Frequently Asked Questions

What is the catalog number for Bulk Anti-Human CD5 (UCHT-2) Antibody?

The catalog number for Bulk Anti-Human CD5 (UCHT-2) Antibody is ICH1174-50mg.

What sizes are available for Bulk Anti-Human CD5 (UCHT-2) Antibody?

Bulk Anti-Human CD5 (UCHT-2) Antibody is available in the following size(s): 50 mg.

Is Bulk Anti-Human CD5 (UCHT-2) Antibody in stock?

Bulk Anti-Human CD5 (UCHT-2) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.