- Catalog number
- ICH1175-25mg
- Size / count
- 25 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform.
- CAS Number
- 9007-83-4
- Specifications
- Clone UCHL-1 recognizes an epitope on human CD45
- Product Name Alternative
- CD45RO molecule is the 180 kDa isoform of CD45 Leucocyte Common Antigen (LCA)
- UniProt
- P08575
- Host
- Mouse
- Species Reactivity
- Human
- Target
- CD45RO
- Isotype
- IgG2a
- Clone
- UCHL-1
- Applications
- CODEX®, Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Western Blot
- Purification Method
- This monoclonal antibody was purified using Protein A
- Concentration
- ≥ 5.0 mg/mL
- Endotoxin
- <1.0 EU/mg as determined by the LAL method
- Purity
- ≥95% monomer by analytical SEC, >95% by SDS Page
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
- Antigen Distribution
- The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive infiltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas. NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes.
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- The suggested concentration for this CD45 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
- Isotype Control
- https://www.ichor.bio/product/Mouse-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-C1.18.4-ICH2245
