Anti-Mouse MHC Class II (M5/114.15.2) In Vivo Antibody - Low Endotoxin

Specifications

Catalog number

ICH1176-5mg

Size / count

5 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

MHC class II antibody, clone M5/114.15.2, recognizes the major histocompatibility complex (MHC) class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. MHC class II is constitutively expressed on professional antigen-presenting cells (APCs), including macrophages/monocytes, dendritic cells (DCs), and B cells, and is induced on T cells upon activation. MHC class II consists of two transmembrane proteins, a 35 kDa α (heavy) chain and 29 kDa β (light) chain. The N-terminal α1 and β1 domains form the antigen-binding groove, which binds 13-25 aa peptides derived from exogenous antigens. On APCs, MHC class II plays a critical role in the adaptive immune response by presenting phagocytosed antigens to helper CD4 T cells. The T cell receptor (TCR)/CD3 complex of CD4 T cells interacts with peptide-MHC class II, which induces CD4 T cell activation leading to the coordination and regulation of other effector cells. CD4 molecules also bind to MHC class II, which helps augment TCR signaling. It has also been demonstrated that MHC class II express on activated T cells are capable of antigen presentation and can transduce signals into T cells, enhancing T cell proliferation and activity.

CAS Number

9007-83-4

Specifications

Clone M5/114.15.2 recognizes an epitope on the mouse MHC class II molecules I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek. Clone M5/114.15.2 does not react with I-Af, I-Ak, or I-As.

Product Name Alternative

I-A/I-E MHC class II

Host

Rat

Species Reactivity

Mouse

Target

MHC Class II

Isotype

IgG2b

Clone

M5/114.15.2

Applications

Blocking, CODEX®, Flow Cytometry, IHC (Frozen), Immunoprecipitation

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

≥ 5.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC, >95% by SDS Page

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Antigen Distribution

I-Ab/d/q and I-Ed/k are expressed on antigen-presenting cells, including macrophages, monocytes, DCs, and B cells, and activated T cells from mice of the I-Ab/d/q and I-Ed/k haplotypes.

Aggregation

Aggregation level ≤ 5%

Isotype Control

https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243