- Catalog number
- ICH1178-25mg
- Size / count
- 25 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- FasL antibody, clone AFS98, recognizes Fas ligand (FasL), also known as CD178, Apo-1 ligand, and CD95 ligand. FasL is a 40 kDa type II integral membrane protein that belongs to the tumor necrosis factor (TNF) superfamily. FasL is expressed by activated T cells and natural killer (NK cells). Binding of FasL to its receptor Fas (CD95, APO-1) induces apoptotic cell death in Fas-expressing target cells, contributing to anti-viral immunity. FasL also contributes to peripheral tolerance and the downregulation of immune responses through activation-induced autocrine and paracrine T cell death. FasL is also found in the anterior chamber of the eye and on Sertoli cells in the testis, and is implicated in immune-privilege at these sites. FasL also contributes to CD8 proliferation and neutrophil recruitment. Soluble FasL (26 kDa) can be released following cleavage by metalloproteinases and block FasL-mediated signaling. Fas/FasL-signaling is involved in the development of many human diseases, including autoimmunity and cancer. Many human tumors over-express FasL, resulting in tumor infiltrating lymphocyte (TIL) apoptosis and immune evasion, which is associated with poor prognosis.
- CAS Number
- 9007-83-4
- Specifications
- Clone MFL3 recognizes an epitope on mouse FasL
- Product Name Alternative
- FasL, Apo-1 Ligand, CD95 Ligand, TNFSF6, CD178
- UniProt
- P41047
- Host
- Armenian Hamster
- Species Reactivity
- Mouse
- Target
- Fas Ligand
- Isotype
- IgG
- Clone
- MFL3
- Applications
- Blocking, Flow Cytometry, Immunofluorescence
- Purification Method
- This monoclonal antibody was purified using Protein A
- Concentration
- ≥ 5.0 mg/mL
- Endotoxin
- <1.0 EU/mg as determined by the LAL method
- Purity
- ≥95% monomer by analytical SEC, >95% by SDS Page
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
- Antigen Distribution
- FasL is expressed on activated T cells, NK cells, the eye, and testis
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- The suggested concentration for this Fas Ligand antibody, clone MFL3, for staining cells in flow cytometry is ≤ 0.25 μg per 10^6 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
- Isotype Control
- https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251
