- Catalog number
- ICH1186-50mg
- Size / count
- 50 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Galectin-9 (Gal-9), a member of the galectin family of mammalian lectins, binds β-galactoside. Gal-9 is expressed by many cell types, including endothelial cells, the epithelium of the gastrointestinal tract, T cells, B cells, macrophages, and mast cells. Gal-9 plays a significant role in innate and adaptive immunity and regulates excessive immunity by suppressing interleukin (IL)-17 producing effector T helper cells (Th)17 and Th1 as well as by augmenting Foxp3+ regulatory T cells (Treg). In addition, Gal-9 induces monocytic myeloid-derived suppressor cells (MDSCs), granulocytic MDSCs, and plasmacytoid dendritic cell-like macrophages. Gal-9 also suppresses B cell receptor signaling and is regulated by I-branching of N-glycans. Additionally, Gal-9 stimulates the maturation of dendritic cells.
Gal-9 is thought to function by binding to specific carbohydrate moieties in receptor molecules expressed on the surface of its target cells, including the T-cell immunoglobulin- and mucin-domain-containing molecule-3 (Tim-3)1. Gal-9 is predominantly located in the cytoplasm but can also be secreted. Gal-9 is expressed by a variety of tumor cells, plays a role in tumor immunity, and can suppress tumor metastasis by blocking adhesion to endothelium and extracellular matrices. CD45, which regulates antigen receptor signaling, is a major glycoprotein receptor for Gal-9 on naive B cells.
- CAS Number
- 9007-83-4
- Specifications
- RG9-1 activity is directed against mouse galectin-9
- Product Name Alternative
- Gal-9
- UniProt
- O08573
- Host
- Rat
- Species Reactivity
- Mouse
- Target
- Galectin-9
- Isotype
- IgG2b Kappa
- Clone
- RG9-1
- Applications
- Blocking
- Purification Method
- This monoclonal antibody was purified using Protein G
- Concentration
- ≥ 5.0 mg/mL
- Endotoxin
- <1.0 EU/mg as determined by the LAL method
- Purity
- ≥95% monomer by analytical SEC, >95% by SDS Page
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
- Antigen Distribution
- Galectin-9 is expressed by many cell types, including T cells, B cells, macrophages, dendritic cells, astrocytes, mast cells, eosinophils, fibroblasts, endothelial cells, and epithelial cells
- Aggregation
- Aggregation level ≤ 5%
- Isotype Control
- https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243
