Bulk Anti-Human CD18 (IB4) Antibody

Catalog number ICH1189-25mg

Price for ICH1189-25mg

Size / count 25 mg

Specifications

Catalog number: ICH1189-25mg
Size / count: 25 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: Murine (m) 1B4 was humanized (h) by the CDR-grafting method to create an anti-CD18 antibody with similar avidity, specificity, and efficacy as the murine antibody. In rhesus monkeys, h1B4 is less immunogenic than m1B4 and elicits only anti-idiotypic antibodies. Additionally, h1B4 and m1B4 exhibit equivalent staining specificity and intensity in rabbit bone marrow leukocytes. For humanization, human VH and VL FR were chosen based on high identity with their corresponding m1B4 FR3. Once grafted with the m1B4 CDR, the human VH and VL FR were expressed as fusion proteins with the IgG4 and Cκ human constant regions, respectively. The h1B4 monoclonal antibody has been used in multiple studies of adhesion in different model systems and to measure the expression of CD18 in B-ALL cell lines.
CAS Number: 9007-83-4
Specifications: Anti-CD18 antibody (clone 1B4) activity is directed against human CD18 and can also stain rabbit bone marrow leukocytes.
Product Name Alternative: Integrin beta-2, integrin beta chain, beta 2, ITGB2
Host: Mouse
Species Reactivity: Human
Target: CD18
Isotype: IgG2a Kappa
Clone: 1B4
Applications: Functional Assays, Flow Cytometry, IF Microscopy
Purification Method: This monoclonal antibody was purified using Protein A
Concentration: ≥ 2.0 mg/mL
Endotoxin: <1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: Sterile, preservative-free, solution in PBS. BSA and Azide free.
Antigen Distribution: Depending on its heterodimeric configuration, CD18 is expressed in leukocytes, monocytes, macrophages, some lymphocytes, polymorphonuclear lymphocytes (PMNs), natural killer cells, neutrophils, granulocytes, certain lymphocytic tumor cell lines, macrophage foam cells, activated platelets, and splenic red pulp macrophages.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Mouse-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-C1.18.4-ICH2245

Frequently Asked Questions

What is the catalog number for Bulk Anti-Human CD18 (IB4) Antibody?

The catalog number for Bulk Anti-Human CD18 (IB4) Antibody is ICH1189-25mg.

What sizes are available for Bulk Anti-Human CD18 (IB4) Antibody?

Bulk Anti-Human CD18 (IB4) Antibody is available in the following size(s): 25 mg.

Is Bulk Anti-Human CD18 (IB4) Antibody in stock?

Bulk Anti-Human CD18 (IB4) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Does this cell line or biologic require a Material Transfer Agreement (MTA)?

Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.