- Catalog number
- ICH1189-50mg
- Size / count
- 50 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Murine (m) 1B4 was humanized (h) by the CDR-grafting method to create an anti-CD18 antibody with similar avidity, specificity, and efficacy as the murine antibody. In rhesus monkeys, h1B4 is less immunogenic than m1B4 and elicits only anti-idiotypic antibodies. Additionally, h1B4 and m1B4 exhibit equivalent staining specificity and intensity in rabbit bone marrow leukocytes.
For humanization, human VH and VL FR were chosen based on high identity with their corresponding m1B4 FR3. Once grafted with the m1B4 CDR, the human VH and VL FR were expressed as fusion proteins with the IgG4 and Cκ human constant regions, respectively.
The h1B4 monoclonal antibody has been used in multiple studies of adhesion in different model systems and to measure the expression of CD18 in B-ALL cell lines.
- CAS Number
- 9007-83-4
- Specifications
- Anti-CD18 antibody (clone 1B4) activity is directed against human CD18 and can also stain rabbit bone marrow leukocytes.
- Product Name Alternative
- Integrin beta-2, integrin beta chain, beta 2, ITGB2
- Host
- Mouse
- Species Reactivity
- Human
- Target
- CD18
- Isotype
- IgG2a Kappa
- Clone
- 1B4
- Applications
- Functional Assays, Flow Cytometry, IF Microscopy
- Purification Method
- This monoclonal antibody was purified using Protein A
- Concentration
- ≥ 2.0 mg/mL
- Endotoxin
- <1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- Sterile, preservative-free, solution in PBS. BSA and Azide free.
- Antigen Distribution
- Depending on its heterodimeric configuration, CD18 is expressed in leukocytes, monocytes, macrophages, some lymphocytes, polymorphonuclear lymphocytes (PMNs), natural killer cells, neutrophils, granulocytes, certain lymphocytic tumor cell lines, macrophage foam cells, activated platelets, and splenic red pulp macrophages.
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Mouse-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-C1.18.4-ICH2245
