- Catalog number
- ICH1190-50mg
- Size / count
- 50 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- KP1 was originally used as a pan-monocytic/macrophage marker against CD68; however, CD68 is not a macrophage specific antigen and KP1 is known to stain neutrophils and other non-macrophage-like cells. KP1 reacts against CD68 in a wide range of healthy and disease-associated (rheumatoid arthritis and osteoarthritis)5 tissues as well as a variety of neoplasms, tumor cell lines, and tumor-associated macrophages. KP1 detects a fixation-resistant epitope that is likely glycan-based which is shared by many cell types.
KP1 was developed by immunizing Balb/c mice against a lysosomal fraction of human lung . Hybridoma supernatants derived from spleen cells were screened on cryostat human lung and tonsil sections as well as paraffin wax sections of lung tissue fixed in formol saline.
- CAS Number
- 9007-83-4
- Specifications
- Anti-CD68 antibody (clone KP1) activity is directed against human CD68.
- Product Name Alternative
- GP110, LAMP4, SCARD1
- Host
- Mouse
- Species Reactivity
- Human
- Target
- CD68
- Isotype
- IgG1 Kappa
- Clone
- KP1
- Applications
- Blotting, Dot Blot, Electron Microscopy, Flow Cytrometry, Immunocytochemistry, ICFC, Immunohistochemistry, IHC-paraffin, Immunoprecipitation, Western Blotting
- Purification Method
- This monoclonal antibody was purified using Protein A
- Concentration
- ≥ 2.0 mg/mL
- Endotoxin
- <1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- Sterile, preservative-free, solution in PBS. BSA and Azide free.
- Antigen Distribution
- CD68 is found in the cytoplasm of monocytes/macrophages, fibroblasts, human peripheral blood lymphocytes, neutrophil primary and mast cell granules, large granular lymphocytes, basophils, basal epithelial cell layers, renal glomeruli, myeloid cells, endothelial cells, retinal epithelial cells, osteoblasts, fibroblast-like cells from bone marrow, and a wide-range of lymphoid neoplasms. CD68 is predominantly located in lysosomal membranes, with a small amount on the cell surface. Additionally, CD68 can be expressed in most hematopoietic cell lines by phorbol-induced differentiation; soluble CD68 can also be found in serum and urine.
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Mouse-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-HKSP-ICH2247
