- Catalog number
- ICH1192-5mg
- Size / count
- 5 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Mouse CD24 is a small 27 amino acid sialoglycoprotein that is anchored to plasma membranes via a glycosyl-phosphatidylinositol linker. CD24 is widely distributed and plays a role in many diverse functions including adaptive immunity, inflammation, autoimmunity, and cancer. CD24 modulates growth and differentiation signals to granulocytes and B cells, is required for homeostatic cell renewal, binds to P-selectin on activated endothelial cells, plays a role in cell adhesion, lymphocyte proliferation for homeostatic purposes4, lymphocyte costimulation via CD28-independent pathways as well as a crucial role in cell selection and maturation during hematopoiesis.
- CAS Number
- 9007-83-4
- Specifications
- Anti-CD24 antibody (clone M1/69) activity is directed against mouse CD24, also known as Heat Stable Antigen (HSA) or Ly-52.
- Product Name Alternative
- Heat Stable Antigen, Nectadrin, Ly-52
- Host
- Rat
- Species Reactivity
- Mouse
- Target
- CD24
- Isotype
- IgG2b Kappa
- Clone
- M1/69
- Applications
- Inhibitor, ELISA Indirect, Flow Cytometry, Immunofluorescence, IF Microscopy, Immunohistochemistry, Immunoprecipitation, Western Blot
- Purification Method
- This monoclonal antibody was purified using Protein G
- Concentration
- ≥ 2.0 mg/mL
- Endotoxin
- <1.0 EU/mg as determined by the LAL method
- Purity
- >95% by SDS-PAGE
- Buffer
- ICH3001-100mL
ICH3002-100mL
ICH3003-100mL
- Shipping Conditions
- Blue ice
- Storage Conditions
- This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
- Formulation
- Sterile, preservative-free, solution in PBS. BSA and Azide free.
- Antigen Distribution
- CD24 is expressed on B cells, T cells, neutrophils, eosinophils, macrophages, neural cells, ganglion cells, keratinocytes, muscle cells, pancreas cells, lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells and is overexpressed in many cancers. Expression varies during T and B cell differentiation. Peripheral T cells are mainly negative.
- Aggregation
- Aggregation level ≤ 5%
- Applications Notes
- Each investigator should determine their own optimal working dilution for specific applications.
- Isotype Control
- https://www.ichor.bio/product/Rat-IgG2b-In-Vivo-Isotype-Control-Low-Endotoxin-1-2-ICH2243