Anti-Mouse CD16.2 (9E9) In Vivo Antibody- Low Endotoxin

Catalog number ICH1194-5mg

Price for ICH1194-5mg

Size / count 5 mg

Specifications

Catalog number: ICH1194-5mg
Size / count: 5 mg
Dry ice: No
Hazardous: No
Discontinued: No
Background: According to surface plasmon resonance, 9E9 has strong reactivity to FcγRIV as well as low level binding to FcγRII and FcγRIII. In vivo, 9E9 binds and blocks FcγRIII only when 9E9 first binds FcγRIV on the same effector cell, resulting in concurrent inhibition of FcγRIII and FcγRIV. Native 9E9 binds to FcγRII and FcγRIII via the Fc. Blocking studies with 9E9 show that FcγRIV is necessary for IgG2a and IgG2b mediated platelet clearance in vivo. Additionally, blocking FcγRIV with 9E9 reduces B-cell depletion. 9E9 also interferes with immune complex binding to FcγRIV and can block FcγRIII on macrophages and neutrophils.
CAS Number: 9007-83-4
Specifications: 9E9 activity is primarily directed against mouse CD16.2 / FcγRIV but can also bind and block FcγRIII in vivo.
Product Name Alternative: FcγRIV
Host: Armenian Hamster
Species Reactivity: Mouse
Target: CD16.2
Isotype: IgG
Clone: 9E9
Applications: Blocking, Flow Cytometry
Purification Method: This monoclonal antibody was purified using Protein A
Concentration: ≥ 2.0 mg/mL
Endotoxin: <1.0 EU/mg as determined by the LAL method
Purity: >95% by SDS-PAGE
Buffer: ICH3001-100mL ICH3002-100mL ICH3003-100mL
Shipping Conditions: Blue ice
Storage Conditions: This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation: Sterile, preservative-free, solution in PBS. BSA and Azide free.
Antigen Distribution: FcγRIV is expressed on the cell membrane of splenic and bone marrow dendritic cells, monocytes, and macrophages as well as peripheral blood monocytes, neutrophils, thioglycollate-elicited macrophages, and myeloid cells. FcγRIV is absent from lymphoid populations, T cells, B cells, NK cells, and other granulocytes.
Aggregation: Aggregation level ≤ 5%
Applications Notes: Each investigator should determine their own optimal working dilution for specific applications.
Isotype Control: https://www.ichor.bio/product/Armenian-Hamster-IgG-Isotype-Control-for-In-Vivo-Low-Endotoxin-PIP-ICH2251

Frequently Asked Questions

What is the catalog number for Anti-Mouse CD16.2 (9E9) In Vivo Antibody- Low Endotoxin?

The catalog number for Anti-Mouse CD16.2 (9E9) In Vivo Antibody- Low Endotoxin is ICH1194-5mg.

What sizes are available for Anti-Mouse CD16.2 (9E9) In Vivo Antibody- Low Endotoxin?

Anti-Mouse CD16.2 (9E9) In Vivo Antibody- Low Endotoxin is available in the following size(s): 5 mg.

Is Anti-Mouse CD16.2 (9E9) In Vivo Antibody- Low Endotoxin in stock?

Anti-Mouse CD16.2 (9E9) In Vivo Antibody- Low Endotoxin is currently made to order / not held in immediate stock. Contact us for lead time.

What should I do if I need to report a performance issue with a specific lot of this product?

Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.

What is the isotype of this antibody?

Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.

Can I get a trial-size or discounted first order of this product?

We don’t generally offer free samples, but trial discounts in exchange for feedback and validation results are almost always possible. Contact us with your intended application to arrange one.