Anti-Mouse CD134 (OX-86) In Vivo Antibody - Low Endotoxin

Specifications

Catalog number

ICH1196-5mg

Size / count

5 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD134 functions as an important immune checkpoint, and its depletion in murine mouse models demonstrate that lack of CD134 expression leads to reduced CD4+ and CD8+ T cells. When CD134 is bound by its corresponding ligand (OX-40L), an optimal T cell response is generated and plays a significant role in determining the amount of memory T-cells remaining after the immune response. CD134 has also been found to play an important role in carcinogenesis, as treatment with activating in vivo antibodies against CD134 enhanced tumor growth, suggesting that CD134 is an important tumor suppressor, and its absence disrupts the immune response to tumors

CAS Number

9007-83-4

Specifications

Clone OX-86 reacts with murine CD134 (OX-40, TNFRSF4).

Product Name Alternative

OX-40, TNFRSF4, ACT35

Host

Rat

Species Reactivity

Mouse

Target

CD134

Isotype

IgG1

Clone

OX-86

Applications

Activation, Flow Cytometry, Immunohistochemistry, Western Blotting

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

≥ 2.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

Sterile, preservative-free, solution in PBS. BSA and Azide free.

Antigen Distribution

CD134 is expressed on activated CD4 and CD8 T cells, activated regulatory T cells, B cells, NKT cells, NK cells, and neutrophils.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG1-Isotype-Control-for-In-Vivo-Low-Endotoxin-GL113-ICH2246