Anti-Mouse CD317 (927) In Vivo Antibody - Low Endotoxin

Specifications

Catalog number

ICH1205-50mg

Size / count

50 mg

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9007-83-4

Specifications

Clone 927 activity is directed against murine CD317 (BST2; PDCA-1).

Host

Rat

Species Reactivity

Mouse

Target

CD317

Isotype

IgG2a kappa

Clone

927

Applications

Depletion, Functional Assays, Flow Cytometry, Immunocytochemistry, Immunofluorescence Microscopy

Purification Method

This monoclonal antibody was purified using Protein G

Concentration

≥ 2.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

Sterile, preservative-free, solution in PBS. BSA and Azide free.

Antigen Distribution

Murine CD317 is expressed by IFN-producing cells in naïve mouse spleen and by a wide variety of cell lines including T cells, mast cells, B cells, fibroblast cells, and pluripotent embryonal carcinoma cells. Additionally, mice challenged by influenza or other stimuli (CpG, LPS, murine CMV, poly(I:C), and imiquimod) express CD317 in DC and other myeloid cells as well as T cells, B cells, NKT cells, and some NK cells. CD317 is also expressed on CD138+ plasma cells in naïve mice and is upregulated by viral stimulation.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.

Isotype Control

https://www.ichor.bio/product/Rat-IgG2a-In-Vivo-Isotype-Control-Low-Endotoxin-1-1-ICH2244