Bulk Human IgG1 (IB1) Isotype Control D265A/N297A

Specifications

Catalog number

ICH2254D265AN297A-100mg

Size / count

100 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Recombinant IgG1 human antibody

Specifications

This Human IgG1 isotype control recombinant antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Furthermore, this low endotoxin Human IgG1 antibody is suitable for in vivo applications and each lot is IMPACT I certified.

Host

CHO

Species Reactivity

Human

Target

IgG1

Isotype

IgG1

Clone

IB1

Applications

Flow Cytometry, Functional Assays

Purification Method

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Concentration

≥ 5.0 mg/mL

Endotoxin

<1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC, >95% by SDS Page

Buffer

ICH3001-100mL ICH3002-100mL ICH3003-100mL

Shipping Conditions

Blue ice

Storage Conditions

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Aggregation

Aggregation level ≤ 5%

Applications Notes

Each investigator should determine their own optimal working dilution for specific applications.