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Product details
Catalog number 52200
Price for 52200
Size / count 10 Preparations
Reduce endotoxin levels to 0.1 EU/µg DNA or less Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume Remove endotoxins from up to 1 mg of DNA with the Maxi format kit Fast and easy processing using a rapid spin-column format These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen's spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample. Endotoxin Removal Kit (Mini) This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes. Endotoxin Removal Kit (Midi) This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes. Endotoxin Removal Kit (Maxi) This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes. About Endotoxins Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.
The catalog number for Endotoxin Removal Kit (Midi) - For DNA is 52200.
Endotoxin Removal Kit (Midi) - For DNA is available in the following size(s): 10 Preparations.
Endotoxin Removal Kit (Midi) - For DNA is currently made to order / not held in immediate stock. Contact us for lead time.
The safety data sheet for Endotoxin Removal Kit (Midi) - For DNA is available here: https://norgenbiotek.com/sites/default/files/resources/22700%20Endotoxin%20Removal%20Kit%20(Mini)%20-%20For%20DNA.pdf.
Reduce endotoxin levels to 0.1 EU/µg DNA or less Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume Remove endotoxins from up to 1 mg of DNA with the Maxi format kit Fast and easy processing using a rapid spin-column format These kits are designed for the rapid spin colum
Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.
Provide the catalogue number, product name, lot number, and your experimental results to our technical support team. They’ll investigate and, if needed, escalate to the manufacturer, provided the product was used before expiry and stored correctly.
Isotype information such as IgG1 or IgG2a is listed in the product specifications on the datasheet, relevant for selecting compatible detection reagents.
