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Product details
Catalog number 46400
Price for 46400
Size / count 250 Preparations
Isolate high quality, high yield plasmid DNA Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli. Plasmid MiniPrep Kit This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format. Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System) Norgen's Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS. Norgen's Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system. Plasmid MaxiPrep Kit This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.
The catalog number for Plasmid MiniPrep Kit-250p is 46400.
Plasmid MiniPrep Kit-250p is available in the following size(s): 250 Preparations.
Plasmid MiniPrep Kit-250p is currently made to order / not held in immediate stock. Contact us for lead time.
The safety data sheet for Plasmid MiniPrep Kit-250p is available here: https://norgenbiotek.com/sites/default/files/resources/46400%20Plasmid%20MiniPrep%20Kit-250p.pdf.
Isolate high quality, high yield plasmid DNA Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPre
Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.
Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.
If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.
