Plasma/Serum Exosome Purification and RNA Isolation Midi Kit

About this product

Purification and enrichment of intact plasma/serum exosomes for functional studies Bind and elute all RNA irrespective of size or GC content, without bias Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias Versatile plasma/serum input volume No phenol extractions, Proteinase K treatment, nor carrier RNA required No time-consuming ultracentrifugation, filtration nor special syringes required No precipitation reagents, nor overnight incubation required Compatible with plasma/serum from any species Pure exosomes are purified and are free-from any other RNA-binding proteins Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Norgen’s Plasma/Serum Exosome and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA and free-circulating RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays. Norgen’s Plasma/Serum Exosome and RNA Isolation Mini Kit For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. Norgen’s Plasma/Serum Exosome and RNA Isolation Midi Kit For sample volumes ranging from 1 mL to 4 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. Norgen’s Plasma/Serum Exosome and RNA Isolation Maxi Kit For sample volumes ranging from 4 mL to 10 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Specifications

Catalog number

58500

Size / count

25 Preparations

Dry ice

No

Hazardous

No

Discontinued

No

MOQ

1

UNSPSC

41116133

Dot Regulated

False

Weight

2

Length

6

Shipping Conditions

Room Temperature

Storage Conditions

RT

Storage Temperature

Room Temperature

Width

10

Height

6

Shelf Life

2 years