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Product details
Catalog number 70500
Price for 70500
Size / count 24 Preparations
Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more Simple and quick workflow: library could be prepared in less than 5 hours Component of Norgen's metagenomics workflow A single NGS run can be prepared with up to 384 unique dual-index libraries The 16S V3-V5 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V3-V5 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others. The 16S V3-V5 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below) . Input DNA is first subjected to targeted PCR to amplify the V3-V4 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5' and 3' barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling, and sequencing.
The catalog number for 16S V3-V5 Library Preparation Kit for Illumina is 70500.
16S V3-V5 Library Preparation Kit for Illumina is available in the following size(s): 24 Preparations.
16S V3-V5 Library Preparation Kit for Illumina is currently made to order / not held in immediate stock. Contact us for lead time.
16S V3-V5 Library Preparation Kit for Illumina is classified as hazardous (additional shipping fees may apply) and requires dry ice shipping.
The safety data sheet for 16S V3-V5 Library Preparation Kit for Illumina is available here: https://norgenbiotek.com/sites/default/files/resources/70500%20-%2016S%20V3-V5%20Library%20Preparation%20Kit%20for%20Illumina%20-%20SDS.pdf.
Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more Simple and quick workflow: library could be prepared in less than 5 hours Component of Norgen's metagenomics workflow A single NGS run can be prepared with up to 384 uni
Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.
Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.
A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.
