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Product details
Catalog number 70800
Price for 70800
Size / count 24 Preparations
Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more Simple and quick workflow: library could be prepared in less than 5 hours Component of Norgen's metagenomics workflow A single NGS run can be prepared with up to 384 unique dual-index libraries The 16S V5-V7 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the 16S V5-V7 amplicon libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal swab, plasma/serum, tongue swab, gum swab, and others. The 16S V5-V7 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below) . Input DNA is first subjected to targeted PCR to amplify the V5-V7 region of the DNA encoding 16S rRNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5' and 3' barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
The catalog number for 16S V5-V7 Library Preparation Kit for Illumina is 70800.
16S V5-V7 Library Preparation Kit for Illumina is available in the following size(s): 24 Preparations.
16S V5-V7 Library Preparation Kit for Illumina is currently made to order / not held in immediate stock. Contact us for lead time.
16S V5-V7 Library Preparation Kit for Illumina is classified as hazardous (additional shipping fees may apply) and requires dry ice shipping.
The safety data sheet for 16S V5-V7 Library Preparation Kit for Illumina is available here: https://norgenbiotek.com/sites/default/files/resources/70800%20-%2016S%20V5-V7%20Library%20Preparartion%20Kit%20for%20Illumina-%20SDS.pdf.
Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more Simple and quick workflow: library could be prepared in less than 5 hours Component of Norgen's metagenomics workflow A single NGS run can be prepared with up to 384 uni
Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.
Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.
A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.
