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Product details
Catalog number 71020
Price for 71020
Size / count 96 Preparations
Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more Simple and quick workflow: library could be prepared in less than 4 hours Component of Norgen's metagenomics workflow A single NGS run can be prepared with up to 384 unique dual-index libraries The ITS1 Library Preparation Kit for Illumina consists of the reagents and components required for library preparation of the fungal target ITS1 libraries to be used for next-generation sequencing on Illumina platforms. All molecular reagents including primers, enzyme mixes, indexes, and buffers are provided. Instructions for PCR clean up with the AMPure XP Magnetic Beads (supplied by customer) are also included for rapid purification of nucleic acid products generated at two steps of the workflow. The library prep workflow could be used for purified DNA inputs from different sources including stool, soil, water, saliva, plant, urine, skin swab, vaginal swab, cheek swab, nasal/swab, plasma/serum, tongue swab, gum swab, and others. The ITS1 Library Preparation Kit for Illumina has a streamlined procedure that reduces the handling time such that the library prep procedure can be completed in approximately 4 hours (see diagram below) . Input DNA is first subjected to targeted PCR to amplify the ITS1 region of the fungal DNA. The post-PCR reaction is then cleaned up using AMPure XP beads. Dual index primers are then added using a limited-cycle PCR. The indexed amplicons flanked by 5' and 3' barcoded adaptors are then cleaned using AMPure XP beads. The libraries are then ready for quantification, pooling and sequencing.
The catalog number for ITS1-ITS2 Library Preparation Kit for Illumina (Set B) is 71020.
ITS1-ITS2 Library Preparation Kit for Illumina (Set B) is available in the following size(s): 96 Preparations.
ITS1-ITS2 Library Preparation Kit for Illumina (Set B) is currently made to order / not held in immediate stock. Contact us for lead time.
ITS1-ITS2 Library Preparation Kit for Illumina (Set B) is classified as hazardous (additional shipping fees may apply) and requires dry ice shipping.
Protocol optimized for DNA isolated from a diversity of samples including stool, soil, water, saliva, plant, urine, skin, and more Simple and quick workflow: library could be prepared in less than 4 hours Component of Norgen's metagenomics workflow A single NGS run can be prepared with up to 384 uni
Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.
Some products, including certain cell lines and biologics, require dry ice for transportation, which carries an additional fee shown clearly in your quotation. Dry ice delivery isn’t always possible to every destination due to transit time or local restrictions, so we check feasibility in advance and you must store the goods at the correct temperature the moment they arrive.
Cross-reactivity data, based on available testing, is listed under the reactivity section of the datasheet. Contact us if you need reactivity confirmed for a species not listed.
