2X One-Step RT-PCR Master Mix-100p

About this product

Convenient ready-to-use solution High sensitivity and yield Robust amplification Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol) This product is also a recommended add-on to be used with Norgen's COVID-19 Primer and Probe Solutions and COVID-19 Positive Control Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products. Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV. Norgen’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes: Cat # 28113 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume) Cat # 28114 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume) Cat # 28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)

Specifications

Catalog number

28113

Size / count

100 Reactions

Dry ice

Yes

Hazardous

Yes

Discontinued

No

MOQ

1

UNSPSC

41106300

UN Code

UN1845

Dot Regulated

True

Weight

28

Length

13

Shipping Conditions

Dry Ice

Storage Conditions

Freezer

Storage Temperature

-20

Width

15

Height

12

Shelf Life

When stored at the proper temperature this reagent is stable for at least 1 year.