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Product details
Catalog number 28115
Price for 28115
Size / count 500 Reactions
Convenient ready-to-use solution High sensitivity and yield Robust amplification Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol) This product is also a recommended add-on to be used with Norgen's COVID-19 Primer and Probe Solutions and COVID-19 Positive Control Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution that contains components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl2, KCl, and a PCR enhancer/stabilizer. The user needs only to add the template, the primer set and water to the Master Mix to set up the RT-PCR reaction. This convenient 2X One-Step RT-PCR Master Mix reduces the time required to set up PCR reactions and reduces the possibility of contamination, particularly when preparing large numbers of reactions. The optimized master mix allows for robust amplification of RNA templates with high yields of PCR products. Taq DNA Polymerase is a highly thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a very low 5´→ 3´ exonuclease activity. The source of Taq included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1. M-MuLV Reverse Transcriptase is an RNA-directed DNA polymerase that can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The source of the Reverse Transcriptase included with Norgen’s 2X One-Step RT-PCR Master Mix is an E. coli strain with a cloned reverse transcriptase gene from M-MuLV. Norgen’s 2X One-Step RT-PCR Master Mix is available in 3 convenient sizes: Cat # 28113 - 100 reactions (sufficient for 100 reactions x 20 µL reaction volume) Cat # 28114 - 200 reactions (sufficient for 200 reactions x 20 µL reaction volume) Cat # 28115 – 500 reactions (sufficient for 500 reactions x 20 µL reaction volume)
The catalog number for 2X One-Step RT-PCR Master Mix-500p is 28115.
2X One-Step RT-PCR Master Mix-500p is available in the following size(s): 500 Reactions.
2X One-Step RT-PCR Master Mix-500p is currently made to order / not held in immediate stock. Contact us for lead time.
2X One-Step RT-PCR Master Mix-500p is classified as hazardous (additional shipping fees may apply) and requires dry ice shipping.
The safety data sheet for 2X One-Step RT-PCR Master Mix-500p is available here: https://norgenbiotek.com/sites/default/files/resources/28115%20-%202x%20One-Step%20RT-PCR%20Master%20Mix%20-%20SDS.pdf.
Convenient ready-to-use solution High sensitivity and yield Robust amplification Available in 3 convenient sizes: 100, 200 and 500 reactions (20 µL vol) This product is also a recommended add-on to be used with Norgen's COVID-19 Primer and Probe Solutions and COVID-19 Positive Control Norgen’s 2X On
Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.
Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.
Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.
