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Product details
Catalog number 28302
Price for 28302
Size / count 1 Unit
Quantified standard to be used as a positive control or PCR quantification standard Vigorously quantified using multiple methods Includes partial sequence of STX-1 subunit A, and STX-2 subunit A genes (please refer to product insert for more details) Compatible with E. coli O157:H7 TaqMan PCR Detection Kit and E. coli O157:H7 TaqMan PCR Detection Probe/Primer and Control Set E. coli O157:H7 is a rod-shaped, gram negative bacterium. It is an enterohemorrhagic strain of the common E. coli bacterium and infection by the O157:H7 strain is commonly associated with hemorrhagic colitis. E. coli O157:H7 is recognized by its somatic (cell wall) antigen (O157) and its flagella antigen (H7) . In addition, E. coli O157:H7 is known to produce Shiga-like toxins, which cause severe symptoms. While most patients can recover from the infection, up to 15% of the patients may develop hemolytic uremic syndrome, a type of kidney failure that could be fatal. Infection of E. coli O157:H7 usually results from consumption of poorly prepared food including undercooked meat (particularly ground beef), untreated water or raw unpasteurized milk. Norgen’s E. coli O157:H7 Quantified Bacterial DNA Standards are cloned fragments of the two Shiga-like toxin regions purified using Norgen’s sample preparation technology. They include partial sequences of STX-1 subunit A gene and STX-2 subunit A gene. Please refer to Appendix A for sequence information. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as positive controls or PCR quantification standards for E. coli O157:H7. This product is compatible with Norgen Biotek's E. coli O157:H7 Taqman PCR Detection Kit (Cat. TM41350) and E. coli O157:H7 Taqman PCR Detection Probe/Primer and Control Set (Cat. TM41310) .
The catalog number for E. coli O157:H7 Quantified Bacterial Standard is 28302.
E. coli O157:H7 Quantified Bacterial Standard is available in the following size(s): 1 Unit.
E. coli O157:H7 Quantified Bacterial Standard is currently made to order / not held in immediate stock. Contact us for lead time.
E. coli O157:H7 Quantified Bacterial Standard is classified as hazardous (additional shipping fees may apply) and requires dry ice shipping.
The safety data sheet for E. coli O157:H7 Quantified Bacterial Standard is available here: https://norgenbiotek.com/sites/default/files/resources/28302%20-%20Escherichia%20coli%20(E%20Coli)%20O157_H7%20Bacterial%20DNA%20Standards%20-%20SDS.pdf.
Quantified standard to be used as a positive control or PCR quantification standard Vigorously quantified using multiple methods Includes partial sequence of STX-1 subunit A, and STX-2 subunit A genes (please refer to product insert for more details) Compatible with E. coli O157:H7 TaqMan PCR Detect
Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.
Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.
Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.
