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Product details
Catalog number 54410
Price for 54410
Size / count 50 Reactions
Convenient One cDNA Synthesis, Multiple microRNAs and microRNA-targets analyzed Time Savings Cost Efficient High Sensitivity and Yield Robust Enzyme Available in 12 or 50 reaction size Norgen's microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations. The kit contains the 2x Reaction Mix and the microScript microRNA Enzyme Mix. The kit utilizes Norgen’s microScript Reverse Transcriptase, a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase. It has reduced RNase H activity and increased thermal stability. The workflow of Norgen's microScript microRNA cDNA Synthesis Kit involves a simple, single-tube set-up by the mixing of 2x Reaction Mix, Enzyme Mix and the RNA template. The reaction can then be carried out in a thermocycler. A poly (A) tail is first added to the RNA template, followed by cDNA synthesis using an adapter primer. In addition to the ease-of-use, the single-tube set-up provides superb consistency and sensitivity. The cDNA could be used in a PCR or qPCR amplification using a Universal PCR Reverse Primer and the forward primer that contains the sequence of the microRNA of interest. A single cDNA preparation could be used for PCR amplification of a number of different microRNAs. In addition, the cDNA preparation could be used for PCR or qPCR detection (using gene-specific forward and reverse primers) of mRNA or large RNA if total RNA preparation was the starting template. This could allow for parallel evaluation of expression level of microRNAs and microRNA-targets.
The catalog number for MicroScript microRNA cDNA Synthesis Kit-50p is 54410.
MicroScript microRNA cDNA Synthesis Kit-50p is available in the following size(s): 50 Reactions.
MicroScript microRNA cDNA Synthesis Kit-50p is currently made to order / not held in immediate stock. Contact us for lead time.
MicroScript microRNA cDNA Synthesis Kit-50p is classified as hazardous (additional shipping fees may apply) and requires dry ice shipping.
The safety data sheet for MicroScript microRNA cDNA Synthesis Kit-50p is available here: https://norgenbiotek.com/sites/default/files/resources/54415%20-%20microScript%20microRNA%20cDNA%20Synthesis%20Kit%20(12%20Reactions)%20-%20SDS.pdf.
Convenient One cDNA Synthesis, Multiple microRNAs and microRNA-targets analyzed Time Savings Cost Efficient High Sensitivity and Yield Robust Enzyme Available in 12 or 50 reaction size Norgen's microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcripti
Suitability for live cell imaging depends on the specific reagent and conjugate used. Check the datasheet for imaging-specific validation, or contact us for guidance.
Yes, we guarantee a full refund or store credit if this product arrives damaged beyond usability, or has a genuine performance issue under proper usage and storage, provided you report it immediately with evidence. Claims after the expiration date or due to improper storage after receipt are excluded.
No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.
