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Product details
Catalog number 54415
Price for 54415
Size / count 12 Reactions
Convenient One cDNA Synthesis, Multiple microRNAs and microRNA-targets analyzed Time Savings Cost Efficient High Sensitivity and Yield Robust Enzyme Available in 12 or 50 reaction size Norgen's microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations. The kit contains the 2x Reaction Mix and the microScript microRNA Enzyme Mix. The kit utilizes Norgen’s microScript Reverse Transcriptase, a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase. It has reduced RNase H activity and increased thermal stability. The workflow of Norgen's microScript microRNA cDNA Synthesis Kit involves a simple, single-tube set-up by the mixing of 2x Reaction Mix, Enzyme Mix and the RNA template. The reaction can then be carried out in a thermocycler. A poly (A) tail is first added to the RNA template, followed by cDNA synthesis using an adapter primer. In addition to the ease-of-use, the single-tube set-up provides superb consistency and sensitivity. The cDNA could be used in a PCR or qPCR amplification using a Universal PCR Reverse Primer and the forward primer that contains the sequence of the microRNA of interest. A single cDNA preparation could be used for PCR amplification of a number of different microRNAs. In addition, the cDNA preparation could be used for PCR or qPCR detection (using gene-specific forward and reverse primers) of mRNA or large RNA if total RNA preparation was the starting template. This could allow for parallel evaluation of expression level of microRNAs and microRNA-targets.
The catalog number for MicroScript microRNA cDNA Synthesis Kit-12p is 54415.
MicroScript microRNA cDNA Synthesis Kit-12p is available in the following size(s): 12 Reactions.
MicroScript microRNA cDNA Synthesis Kit-12p is currently made to order / not held in immediate stock. Contact us for lead time.
MicroScript microRNA cDNA Synthesis Kit-12p is classified as hazardous (additional shipping fees may apply) and requires dry ice shipping.
The safety data sheet for MicroScript microRNA cDNA Synthesis Kit-12p is available here: https://norgenbiotek.com/sites/default/files/resources/54410%20-%20microScript%20microRNA%20cDNA%20Synthesis%20Kit%20(50%20Reactions)%20-%20SDS.pdf.
Convenient One cDNA Synthesis, Multiple microRNAs and microRNA-targets analyzed Time Savings Cost Efficient High Sensitivity and Yield Robust Enzyme Available in 12 or 50 reaction size Norgen's microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcripti
Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.
Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.
Some products are subject to special import/export regulations, dangerous goods handling, or licensing requirements. Where this applies, it’s noted on the product page, and any required extra documents or fees will be confirmed with you before dispatch, never added without your consent.
