Product details
Catalog number TM33150
Price for TM33150
Size / count 100 Reactions
Detection kits for Mycoplasma DNA Available in TaqMan format for analysis Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of cell cultures may be contaminated with Mycoplasmas, the main contaminants being the species M. orale, M. laidlawii, M. arginini and M. hyorhinis. These organisms produce a variety of effects on the infected cells in culture, including changes in metabolism growth, viability and morphology, thereby altering the phenotypic characteristics of the host cells. Therefore there is an absolute requirement for routine, periodic assays to diagnose possible Mycoplasma contamination of all cell cultures, particularly continuous or established cell lines. Mycoplasma TaqMan PCR Kit, 100 reactions Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality Specific Primer and Probe mix for the pathogen/virus/viroid of interest Primer and Probe mix Positive and negative control to confirm the integrity of the kit reagents Mycoplasma TaqMan PCR Probe/Primer Set and Controls, 100 reactions Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest Nuclease-free water Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix For research use only and NOT intended for in vitro diagnostics.
The catalog number for Mycoplasma TaqMan PCR Kit is TM33150.
Mycoplasma TaqMan PCR Kit is available in the following size(s): 100 Reactions.
Mycoplasma TaqMan PCR Kit is currently made to order / not held in immediate stock. Contact us for lead time.
Mycoplasma TaqMan PCR Kit is classified as hazardous (additional shipping fees may apply) and requires dry ice shipping.
The safety data sheet for Mycoplasma TaqMan PCR Kit is available here: https://norgenbiotek.com/sites/default/files/resources/TM33150%20-%20Mycoplasma%20Taqman%20PCR%20Detection%20Kits%20-%20SDS.pdf.
Detection kits for Mycoplasma DNA Available in TaqMan format for analysis Mycoplasma is a common and serious contaminant of cell cultures. Mycoplasma is often not visible and does not respond to antibiotics, and therefore it is a major issue that requires monitoring and early detection. Up to 30% of
Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.
A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.
Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.