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Product details
Catalog number 30300
Price for 30300
Size / count 25 Preparations
Simple protocol that is free of detergents Fast processing time Process a variety of inputs Compatible with mass spectrometry and NMR spectroscopy Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix This kit provides a fast and simple procedure for the isolation of total proteins from tissue, bacteria, yeast or mammalian cells without the use of SDS, Triton® X-100 and other detergents. Detergents are extensively used to prepare protein samples; however, these detergents have undesirable effects on downstream analysis. These effects include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization. The Detergent-Free Total Protein Isolation Kit maintains high protein recovery and yields proteins that are 100% detergent-free. Purification is based on using Norgen’s proprietary resin together with Lysis Solution, followed by protein filtration using a filter column (provided) . The purified proteins can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, NMR spectroscopy and more. Each Lysis Tube is able to process up to 50 mg of tissue, 1010 bacterial cells, 109 yeast cells or 107 mammalian cells. Preparation time for 12 samples is less than 10 minutes.
The catalog number for Detergent-Free Total Protein Isolation Kit is 30300.
Detergent-Free Total Protein Isolation Kit is available in the following size(s): 25 Preparations.
Detergent-Free Total Protein Isolation Kit is currently made to order / not held in immediate stock. Contact us for lead time.
The safety data sheet for Detergent-Free Total Protein Isolation Kit is available here: https://norgenbiotek.com/sites/default/files/resources/30300%20-%20Detergent%20Free%20Protein%20Isolation%20Kit%20-%20SDS.pdf.
Simple protocol that is free of detergents Fast processing time Process a variety of inputs Compatible with mass spectrometry and NMR spectroscopy Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix This kit provides a fast and simple procedure
We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.
Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.
Shipping temperature is chosen based on the product’s stability needs, ranging from ambient to dry ice, and is indicated on the product page. Any related shipping fee is shown clearly in your quotation.
