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Product details
Catalog number 17500
Price for 17500
Size / count 25 Preparations
Rapid and simple procedure to generate digested peptides Simultaneous digestion, purification and concentration at once Peptide generation is complete, with no generation of additional artifacts being detected in mass spectrometry Peptides are ready for applications such as mass spectrometry and SDS-PAGE Purification is based on spin column chromatography that uses Norgen’s resin separation matrix This kit is highly efficient in the enzymatic digestion of simple and complex protein samples using trypsin and the subsequent purification of the resulting peptides using a convenient spin column format. Trypsin is added to the protein sample and bound to the column. Salts are washed away and the trypsin is then activated to digest proteins. Peptides are then eluted in a small volume and ready for downstream analysis. The peptides generated are complete, with no additional artifacts being detected in mass spectrometry. Fifteen micrograms of protein can be processed, digested and purified with each spin column with about 20 minutes of hands-on time (plus trypsin incubation) . The simultaneous protein digestion and volumetric concentration of the purified peptides makes the kit a convenient method for preparing peptides to be analyzed by many downstream applications such as mass spectrometry and more.
The catalog number for On-Column Proteolytic Digestion Kit is 17500.
On-Column Proteolytic Digestion Kit is available in the following size(s): 25 Preparations.
On-Column Proteolytic Digestion Kit is currently made to order / not held in immediate stock. Contact us for lead time.
The safety data sheet for On-Column Proteolytic Digestion Kit is available here: https://norgenbiotek.com/sites/default/files/resources/17500%20On-Column%20Proteolytic%20Digestion%20Kit%20(25%20preps).pdf.
Rapid and simple procedure to generate digested peptides Simultaneous digestion, purification and concentration at once Peptide generation is complete, with no generation of additional artifacts being detected in mass spectrometry Peptides are ready for applications such as mass spectrometry and SDS
The formulation or storage buffer is listed on the product datasheet. If you require a carrier-free or custom formulation, contact us before ordering, as this may involve a made-to-order production timeline.
A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.
Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.
