FFPE RNA Purification 96-Well Kit

About this product

Extract total RNA (including microRNA) from FFPE samples No phenol extraction step Includes DNase for optional on-column DNA removal Isolated RNA is of the highest quality and integrity Isolate a diversity of RNA species Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform. FFPE RNA Purification Kit (Spin Column) Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column. FFPE RNA Purification 96-Well Kit (High Throughput) Purification is based on 96-well column chromatography using Norgen’s proprietary resin as the separation matrix. Purification can be performed using either a vacuum manifold or centrifugation. Maximum loading volume of 400 μL per well, and a maximum binding capacity of 50 μg per well.

Specifications

Catalog number

25400

Size / count

2 Plates

Dry ice

No

Hazardous

No

Discontinued

No

MOQ

1

UNSPSC

41105518

Dot Regulated

False

Weight

2

Length

6

Shipping Conditions

Room Temperature

Storage Conditions

RT

Storage Temperature

Room Temperature

Width

10

Height

6

Shelf Life

1 year