Product details
Catalog number 73310
Price for 73310
Size / count 25 Preparations
Purification and enrichment of intact cell culture media exosomes for functional studies. No phenol extractions, Proteinase K treatment, nor carrier RNA required. No time-consuming ultracentrifugation, filtration nor special syringes required. No precipitation reagents nor overnight incubation required. Pure exosomes are purified and are free-from any other RNA-binding proteins. Purification is based on EXTRAClean spin column chromatography that uses Norgen’s proprietary resin separation matrix. Norgen’s EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the subsequent isolation of RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The EXTRAClean columns undergo stringent processing and rigorous quality control measures to minimize contamination traces, ensuring optimal results for sensitive applications such as NGS. The kit is designed to isolate all sizes of RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allows the user to elute into flexible elution volumes ranging from 50 μL to 100 μL. The purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, Sequencing based applications, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
The catalog number for EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Midi Kit is 73310.
EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Midi Kit is available in the following size(s): 25 Preparations.
EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Midi Kit is currently made to order / not held in immediate stock. Contact us for lead time.
The safety data sheet for EXTRAClean Cell Culture Media Exosome Purification and RNA Isolation Midi Kit is available here: https://norgenbiotek.com/sites/default/files/resources/73310%20-%20EXTRAClean%20Cell%20Culture%20Media%20%20Exosome%20Purification%20and%20RNA%20Isolation%20Midi%20Kit%20-%20SDS.pdf.
Purification and enrichment of intact cell culture media exosomes for functional studies. No phenol extractions, Proteinase K treatment, nor carrier RNA required. No time-consuming ultracentrifugation, filtration nor special syringes required. No precipitation reagents nor overnight incubation requi
A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.
Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.
Some products, including certain cell lines, plasmids, and tissue cultures, are subject to intellectual property terms and may require an MTA before we can ship. We’ll inform you in advance if this product requires one, and additional handling fees may apply.