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Product details

HisSep Ni-NTA Agarose Resin

Catalog number 20502ES50

Discontinued

Price for 20502ES50

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Size / count 50 mL

This variant is discontinued and cannot be added to your cart.

About this product

HisSep Ni-NTA Agarose Resin is based on a cross-linked 6% agarose gel matrix, chemically coupled with tetradentate nitrilotriacetic acid (NTA) ligands. Upon chelation with nickel ions (Ni2+), it forms a highly stable octahedral structure, with nickel ions situated at the center of the octahedron. This structure effectively shields nickel ions from attack by small molecules, making it more stable compared to Ni-IDA resin. It can withstand harsh conditions including certain concentrations of reducing agents, denaturants, or coupling agents. It has become an indispensable resin for purifying His-tagged proteins in laboratory experiments.

Specifications

Catalog number
20502ES50
Size / count
50 mL
Dry ice
No
Hazardous
No
Discontinued
Yes
Field of Research
Protein Research
Shipping Conditions
Blue Ice

Frequently Asked Questions

What is the catalog number for HisSep Ni-NTA Agarose Resin?

The catalog number for HisSep Ni-NTA Agarose Resin is 20502ES50.

Is HisSep Ni-NTA Agarose Resin in stock?

HisSep Ni-NTA Agarose Resin is currently made to order / not held in immediate stock. Contact us for lead time.

What is HisSep Ni-NTA Agarose Resin used for?

HisSep Ni-NTA Agarose Resin is based on a cross-linked 6% agarose gel matrix, chemically coupled with tetradentate nitrilotriacetic acid (NTA) ligands. Upon chelation with nickel ions (Ni2+), it forms a highly stable octahedral structure, with nickel ions situated at the center of the octahedron. Th

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

How should this product be stored?

Storage conditions depend on the specific reagent and are listed on the product datasheet, with most antibodies and proteins requiring -20°C unless stated otherwise. It is your responsibility to store the product correctly immediately upon receipt; damage from improper storage after delivery is not eligible for replacement.

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