Plant Preservative Mixture (PPM™)

About this product

Plant Preservative Mixture (PPM™), is a broad-spectrum biocide/ for plant tissue culture. PPM™ is the ultimate solution to the never-ending struggle against microbial airborne, waterborne, and endogenous contamination. Coupled with proper observation of aseptic laboratory techniques, PPM™ can increase your chances of a successful and thriving culture experiment.

Specifications

Catalog number

250 PPM

Size / count

250 mL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

PPM™

HS Code

3808.94

Applications

Controller of microbial airborne contamination. Manager of microbial waterborne contamination. Inhibitor of endogenous contamination. Enhancer of the success of culture experiments. Maintainer of the viability of in vitro seed germination. Promoter of callus proliferation. Supporter of callus regeneration. Ensurer of the stability of tissue culture media.

Assay Protocol

General Dosage levels: With the exception of endogenous contamination, the recommended dose range is 0.05%-0.2%. (For callus proliferation, organogenesis and embryogenesis, the recommended range is 0.05-0.075%.) To eliminate higher endogenous contamination densities, higher doses of PPM™ are needed. Endogenous Contamination: (a) For explants: gently and routinely shake / stir 1 cm. long explants (or shorter) for 4-12 hours in 4-5% v/v PPM™ solution supplemented as above with 3x MS strength basal salts without Tween 20.Without rinsing, insert into a medium supplemented with 0.05 - 0.1% PPM™ for herbaceous plants and 0.2% PPM™ for woody plants. Note: Paragraphs 6(b) through 10 below are intended for ornamental plants only. (b)For tubers, bulbs and scales: shake / stir the entire tuber/ bulb / scale in bleach. Rinse with water (can be done under non-sterile conditions). Slice the tuber / bulb /scale to thin slices. Shake / stir for 12-24 hours in 4- 5% PPM™ solution supplemented with full strength basal salts without pH ing & Tween 20. Without rinsing, insert into a medium supplemented with 0.1 -0.2% PPM™.

Capacity

Available in different volumes of 30 mL, 100 mL, 250 mL, 500 mL, and 1L.

Format

Cylindrical container

pH

5 - 7

Material

Liquid

Color

Clear to light amber

Solubility

Infinite in water

Features & Benefits

At optimum doses, PPM™, which stands for Plant Preservative Mixture™, is an extremely effective Preservative/Biocide, yet does not impair in vitro seed germination, callus proliferation and callus regeneration. PPM™ prevents the germination of both bacteria and fungi spores. It is heat stable and therefore can be autoclaved with the media. PPM™ can be, and should be used as a standard ingredient in plant tissue culture media, and is also substantially less expensive than commonly used antibiotics. While PPM™ was principally designed to inhibit airborne contamination, waterborne contamination, and contamination introduced from human contact, it can also, in many cases, be used to reduce endogenous contamination. PPM™ does not affect the genetic profile of the plants.

Storage Temperature

39° F (4° C).

Shelf Life

Check the product label for expiration date

Suitability

For Tissue Culture

Odor

Mild