• Certified Lab Equipment & Reagents
  • Fast Dispatch on In-Stock Items
  • Expert Scientific Support

Product details

DirectPCR Lysis Reagent (Cell)

Catalog number 301-C

Price for 301-C

Sign In for Pricing

Size / count 50 mL

About this product

<font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">DirectPCR Lysis Reagent (Cell) is a molecular biology reagent designed for rapid preparation of DNA templates from cultured cells for direct PCR amplification.</font><div style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">This cell lysis reagent enables simplified DNA preparation by lysing biological samples while maintaining DNA compatibility with downstream PCR workflows, eliminating the need for conventional DNA purification steps.</font></div><div style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><br style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"></font></div><div style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#042283" size="5" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">DirectPCR Cell Lysis Technology Principle </font></span><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">DirectPCR Lysis Reagent (Cell) uses a simplified lysis approach to release genomic DNA from cultured cells for PCR amplification. The reagent helps disrupt cellular components while reducing the impact of PCR inhibitors commonly found in crude biological extracts, allowing the prepared DNA template to be used directly in compatible PCR workflows.</font></div><div style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><br style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"></font></div><div style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><font size="5" color="#042283" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;">Product Highlights </span></font><font color="#042f93" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-size: medium;"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;">✓ </span></font><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">Designed for direct PCR from cultured cells. </font><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder; color: rgb(4, 47, 147); font-size: medium;">✓ </span><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">Rapid cell lysis and DNA preparation. </font><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder; color: rgb(4, 47, 147); font-size: medium;">✓ </span><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">Compatible with PCR genotyping workflows. </font><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder; color: rgb(4, 47, 147); font-size: medium;">✓ </span><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">Minimizes DNA purification steps. </font><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder; color: rgb(4, 47, 147); font-size: medium;">✓ </span><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">Reduces sample handling and transfer steps. </font><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder; color: rgb(4, 47, 147); font-size: medium;">✓ </span><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">Suitable for molecular biology applications.</font></div><div style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><br style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"></font></div><div style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#042283" size="5" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">Applications of DirectPCR Lysis Reagent (Cell) </font></span><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"> This reagent is suitable for: </font><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-size: medium;"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#042f93" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">✓ </font></span></span><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#042f93" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">PCR Genotyping </font></span>Preparation of DNA templates from cultured cells for genotyping analysis. </font><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-size: medium;"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#042f93" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">✓ </font></span></span><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#042f93" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">Molecular Biology Research </font></span>Supporting routine PCR-based experiments. </font><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-size: medium;"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#042f93" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">✓ </font></span></span><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#042f93" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">Cell-Based Studies </font></span>Rapid DNA preparation from mammalian cultured cells. </font><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-size: medium;"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#042f93" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">✓ </font></span></span><font size="3" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#042f93" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">Genetic Analysis </font></span>Streamlining workflows requiring PCR amplification.</font></div>

Specifications

Catalog number
301-C
Size / count
50 mL
Dry ice
No
Hazardous
No
Discontinued
No
Shipping Conditions
Ice Packs
FAQ
<span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder; font-size: medium;"><i style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">What is DirectPCR Lysis Reagent? </font></i><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=&gt; </font></span><span style="font-size: medium;">DirectPCR Lysis Reagent is a cell lysis reagent used to prepare DNA templates directly for PCR amplification without conventional DNA purification. </span><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder; font-size: medium;"><i style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);"><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">What samples are compatible with DirectPCR Cell reagent? </font></i></span><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-size: medium; font-weight: bolder;"><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=&gt; </font></span><span style="font-size: medium;">DirectPCR Lysis Reagent (Cell) is designed for cultured cell samples. </span><i style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-size: medium;"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">Can DirectPCR Lysis Reagent be used for PCR? </font></span></i><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-size: medium; font-weight: bolder;"><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=&gt; </font></span><span style="font-size: medium;">Yes. The reagent is designed to prepare DNA suitable for direct PCR applications. </span><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-size: medium;"><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-weight: bolder;"><i style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">What is the advantage of direct PCR preparation? </i></span></font><span style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5); font-size: medium; font-weight: bolder;"><font color="#042293" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=&gt; </font></span><span style="font-size: medium;">Direct PCR preparation reduces workflow time by combining sample lysis and DNA preparation in fewer steps compared with traditional extraction methods.</span>

Frequently Asked Questions

What is the catalog number for DirectPCR Lysis Reagent (Cell)?

The catalog number for DirectPCR Lysis Reagent (Cell) is 301-C.

What sizes are available for DirectPCR Lysis Reagent (Cell)?

DirectPCR Lysis Reagent (Cell) is available in the following size(s): 50 mL.

Is DirectPCR Lysis Reagent (Cell) in stock?

DirectPCR Lysis Reagent (Cell) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures DirectPCR Lysis Reagent (Cell)?

DirectPCR Lysis Reagent (Cell) is manufactured by Viagen.

What is the estimated delivery time for DirectPCR Lysis Reagent (Cell)?

Estimated delivery time for DirectPCR Lysis Reagent (Cell) is approximately 4 business day(s), depending on your location.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

Is this product classed as hazardous or restricted for shipping?

Some products are subject to special import/export regulations, dangerous goods handling, or licensing requirements. Where this applies, it’s noted on the product page, and any required extra documents or fees will be confirmed with you before dispatch, never added without your consent.

You Might Also Like
CD3e (CRIS-7), CF647 conjugate, 0.1mg/mL
CD3e (CRIS-7), CF647 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC471050-100
Cytokeratin 8/18 (C-51), CF568 conjugate, 0.1mg/mL
Cytokeratin 8/18 (C-51), CF568 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC680034-500
Major Vault Protein (MVP) (1032), CF488A conjugate, 0.1mg/mL
Major Vault Protein (MVP) (1032), CF488A conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC880225-500
Complement C4d (C4D204), CF568 conjugate, 0.1mg/mL
Complement C4d (C4D204), CF568 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC680204-100
P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL
P27 / KIP1 (SX53G8), CF405S conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC040669-500
CD71 (66IG10), CF647 conjugate, 0.1mg/mL
CD71 (66IG10), CF647 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC470871-500
Cytokeratin, LMW (AE-1), CF647 conjugate, 0.1mg/mL
Cytokeratin, LMW (AE-1), CF647 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC470253-500
CD47 (B6H12.2), CF740 conjugate, 0.1mg/mL
CD47 (B6H12.2), CF740 conjugate, 0.1mg/mL
By Afraxis
Sign In for Pricing
Ref: BNC740437-100