DirectPCR Lysis Reagent (Ear)

Catalog number 401-E

Price for 401-E

Size / count 25 mL

About this product

DirectPCR Lysis Reagent (Ear) is a molecular biology reagent for rapid DNA preparation from mouse ear tissue for direct PCR and genotyping. The single-tube DirectPCR® workflow enables preparation of PCR-compatible crude DNA lysates while reducing conventional DNA extraction and sample transfer steps. What is DirectPCR Lysis Reagent (Ear) | Mouse DNA PCR Genotyping? DirectPCR Lysis Reagent (Ear) is designed for the preparation of DNA from mouse ear pieces and ear-punch samples prior to PCR-based genotyping. The reagent combines tissue lysis with a formulation intended to reduce the inhibitory effects of crude biological lysates on PCR, allowing the resulting lysate to be used as a DNA template in compatible PCR workflows. Key Features → Designed for mouse ear tissue and ear-punch samples. → Suitable for direct PCR and genotyping. → Single-tube DNA preparation workflow. → Reduces sample transfer and DNA purification steps. → Designed to produce PCR-compatible crude DNA lysates. → No organic extraction reagents required. → 25 mL format for approximately 250 mouse ear pieces.

Specifications

Catalog number: 401-E
Size / count: 25 mL
Dry ice: No
Hazardous: No
Discontinued: No
Applications: <ul><li>PCR Genotyping → Preparation of DNA templates from mouse ear and ear-punch samples for genomic PCR genotyping. </li><li>Mouse Genetic Screening → Supports screening of genetically engineered mouse colonies and identification of genotypes from ear-punch tissue. </li><li>Molecular Biology Research → Suitable for research workflows requiring rapid preparation of DNA from mouse tissue for downstream PCR analysis.</li></ul><div> </div>Research Use : DirectPCR lysis-based approaches have been used in published research for mouse ear tissue lysis and PCR-based genotyping, supporting the application of rapid crude DNA preparation in mouse genetic screening workflows.
Shipping Conditions: Ice Packs
FAQ: What is DirectPCR Lysis Reagent (Ear)? <h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> DirectPCR Lysis Reagent (Ear) is a PCR lysis reagent designed to prepare DNA from mouse ear tissue for direct PCR and genotyping.</h3> What samples can be used with DirectPCR Ear Reagent? <h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> The reagent is designed for mouse ear pieces and ear-punch tissue used in PCR-based genotyping workflows.</h3> Can DirectPCR Ear Reagent be used for PCR genotyping? <h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> Yes. The resulting crude DNA lysate is designed for use as a template in compatible genomic PCR genotyping workflows.</h3> Does DirectPCR Ear Reagent include Proteinase K? <h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> No. Proteinase K is required for the manufacturer's ear-tissue protocol but is not included with the DirectPCR Lysis Reagent.</h3> What is the capacity of the 25 mL format? <h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer" style="border-color: oklch(0.922 0 0); outline-color: oklab(0.708 0 0 / 0.5);">=> The 0388-401-E 25 mL format is the smaller DirectPCR Ear reagent configuration. If the manufacturer has not explicitly specified the number of ear pieces for this size, I recommend not stating a numerical capacity on the product page.</h3>

Frequently Asked Questions

What is the catalog number for DirectPCR Lysis Reagent (Ear)?

The catalog number for DirectPCR Lysis Reagent (Ear) is 401-E.

What sizes are available for DirectPCR Lysis Reagent (Ear)?

DirectPCR Lysis Reagent (Ear) is available in the following size(s): 25 mL.

Is DirectPCR Lysis Reagent (Ear) in stock?

DirectPCR Lysis Reagent (Ear) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures DirectPCR Lysis Reagent (Ear)?

DirectPCR Lysis Reagent (Ear) is manufactured by Viagen.

What is the estimated delivery time for DirectPCR Lysis Reagent (Ear)?

Estimated delivery time for DirectPCR Lysis Reagent (Ear) is approximately 4 business day(s), depending on your location.

Do you offer custom or private-label production of this type of product?

Yes, custom antibody development and private-label production services are available. Contact our sales team to discuss your target, timeline, and quantity requirements.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.