DirectPCR Lysis Reagent (Ear)

Catalog number 402-E

Price for 402-E

Size / count 50 mL

About this product

DirectPCR Lysis Reagent (Ear) is a molecular biology reagent for rapid DNA preparation from mouse ear tissue for direct PCR and genotyping. The single-tube DirectPCR workflow enables preparation of PCR-compatible crude DNA lysates while reducing conventional DNA extraction and transfer steps.<div> </div><div>What is DirectPCR Lysis Reagent (Ear) | Mouse DNA PCR Genotyping?</div><div>DirectPCR Lysis Reagent (Ear) is designed for preparation of DNA from mouse ear pieces prior to PCR-based genotyping. The reagent combines tissue lysis with a formulation intended to reduce the inhibitory effects of crude biological lysates on PCR, allowing the resulting lysate to be used as a DNA template in compatible PCR workflows.</div><div> </div><div>Key Features => Designed for mouse ear tissue and ear-punch samples. => Suitable for direct PCR and genotyping. => Single-tube DNA preparation workflow => Reduces sample transfer and DNA purification steps. => Designed to produce PCR-compatible crude DNA lysates. => No organic extraction reagents required. => 50 mL format for approximately 500 mouse ear pieces. The manufacturer's current specification states 50 mL / 500 ear pieces for 0388-402-E.</div>

Specifications

Catalog number: 402-E
Size / count: 50 mL
Dry ice: No
Hazardous: No
Discontinued: No
Applications: PCR Genotyping => Preparation of DNA templates from mouse ear samples for genomic PCR genotyping. Mouse Genetic Screening => Useful for screening mouse colonies and identifying genotypes from ear-punch tissue. Molecular Biology Research => Suitable for research workflows requiring rapid DNA preparation from animal tissue. There is also real published-use evidence: research papers have used DirectPCR Ear reagent to lyse mouse ear tissue before PCR-based genotyping. PubMed Central PMCID : PMC11159904
Shipping Conditions: Ice Packs
FAQ: <h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer">What is DirectPCR® Lysis Reagent (Ear)? => DirectPCR® Lysis Reagent (Ear) is a PCR lysis reagent designed to prepare DNA from mouse ear tissue for direct PCR and genotyping. What samples can be used with DirectPCR Ear reagent? </h3><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer">=> The reagent is designed for mouse ear pieces and ear-punch tissue. Can DirectPCR Ear reagent be used for PCR genotyping? </h3><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer">=> Yes. The resulting crude DNA lysate is designed for use as a template in compatible genomic PCR genotyping workflows. Does DirectPCR Ear include Proteinase K? </h3><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer">=> No. Proteinase K is required for the manufacturer's ear-tissue protocol but is not included with the lysis reagent. What is the capacity of the 50 mL format? </h3><h3 data-section-id="qtp2pa" data-start="5847" data-end="5890" class="PDq2pG_selectionAnchorContainer">=> The 0388-402-E 50 mL format is specified for approximately 500 mouse ear pieces.</h3>

Frequently Asked Questions

What is the catalog number for DirectPCR Lysis Reagent (Ear)?

The catalog number for DirectPCR Lysis Reagent (Ear) is 402-E.

What sizes are available for DirectPCR Lysis Reagent (Ear)?

DirectPCR Lysis Reagent (Ear) is available in the following size(s): 50 mL.

Is DirectPCR Lysis Reagent (Ear) in stock?

DirectPCR Lysis Reagent (Ear) is currently made to order / not held in immediate stock. Contact us for lead time.

Who manufactures DirectPCR Lysis Reagent (Ear)?

DirectPCR Lysis Reagent (Ear) is manufactured by Viagen.

What is the estimated delivery time for DirectPCR Lysis Reagent (Ear)?

Estimated delivery time for DirectPCR Lysis Reagent (Ear) is approximately 4 business day(s), depending on your location.

What documentation should I request if this product is used for a regulated or intellectual-property-sensitive application?

If the product is subject to intellectual property protections, we’ll advise on any MTA or usage restrictions before shipping. Let us know your intended end use so we can confirm what’s required upfront.

Is this antibody conjugated?

Conjugation status, such as unconjugated, HRP, FITC, or biotin, is specified in the product name and confirmed on the datasheet.

What is the sensitivity of this ELISA kit?

Assay sensitivity, including the minimum detectable dose, is listed on the datasheet. Kits that are assembled on order typically ship within 2-4 weeks.