ATOX1 Recombinant Rabbit Monoclonal Antibody [PSH08-50]

About this product

ATOX1 is a copper metallochaperone protein that is encoded by the ATOX1 gene in humans. In mammals, ATOX1 plays a key role in copper homeostasis as it delivers copper from the cytosol to transporters ATP7A and ATP7B. Homologous proteins are found in a wide variety of eukaryotes, including Saccharomyces cerevisiae as ATX1, and all contain a conserved metal binding domain. ATOX1 is an abbreviation of the full name Antioxidant Protein 1. The nomenclature stems from initial characterization that showed that ATOX1 protected cells from reactive oxygen species. Since then, the primary role of ATOX1 has been established as a copper metallochaperone protein found in the cytoplasm of eukaryotes. A metallochaperone is an important protein that has metal trafficking and sequestration roles. As a metal sequestration protein, ATOX1 is capable of binding free metals in vivo, in order to protect cells from generation of reactive oxygen species and mismetallation of metalloproteins. As a metal trafficking protein, ATOX1 is responsible for shuttling copper from the cytosol to ATPase transporters ATP7A and ATP7B that move copper to the trans-Golgi network or secretory vesicles. In Saccharomyces cerevisiae, Atx1 delivers Cu (I) to a homologous transporter, Ccc2. The delivery of copper to ATPase transporters is vital for the subsequent insertion of copper into ceruloplasmin, a ferroxidase required for iron metabolism, within the golgi apparatus. In addition to the metallochaperone function, recent reports have characterized ATOX1 as a cyclin D1 transcription factor.

Specifications

Catalog number

HA722996

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

ATOX1 antibody ATOX1_HUMAN antibody ATX1 antibody ATX1 antioxidant protein 1 homolog (yeast) antibody ATX1 antioxidant protein 1 homolog antibody Copper transport protein antibody Copper transport protein ATOX1 antibody HAH1 antibody Metal transport protein antibody Metal transport protein ATX1 antibody MGC138453 antibody MGC138455 antibody

Abbreviation

Copper transport protein ATOX1, Metal transport protein ATX1, ATOX1, HAH1

Swiss Prot

O00244 Human

Cellular Locus

Cytosol.

Host

Rabbit

Species Reactivity

Human

Immunogen

Recombinant protein within human ATOX1 aa 1-68.

Isotype

IgG

Clone

PSH08-50

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC

Positive Control

HeLa cell lysate, 293T cell lysate, MCF7 cell lysate, NCI-H1299 cell lysate, 293T, human liver cancer tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:200 ;FC: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 7 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes