KDM2B Recombinant Rabbit Monoclonal Antibody [PSH05-38]

About this product

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined. As part of the ncPRC1.1 complex, KDM2B was found to be rapidly and transiently recruited to sites of DNA damage in a PARP1- and TIMELESS-dependent manner to promote mono-ubiquitylation of histone H2A on K119 with concomitant local decrease of H2A levels and an increase of H2A.Z. These events promote transcriptional repression at DNA lesions, double strand break signaling, and homologous recombination repair. The activity of the ncPRC1.1 complex at DNA lesions was necessary for the proper recruitment of the two canonical PRC1 complexes (cPRC1.2 and cPRC1.4), defined by their PCGF subunits, MEL18 and BMI1 respectively. Therefore, recruitment of the ncPRC1.1 complex represents an early and critical regulatory step in homologous recombination repair.

Specifications

Catalog number

HA722267

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

[Histone-H3]-lysine-36 demethylase 1B antibody CXXC-type zinc finger protein 2 antibody CXXC2 antibody F box and leucine rich repeat protein 10 antibody F box protein FBL10 antibody F-box and leucine-rich repeat protein 10 antibody F-box protein FBL10 antibody F-box/LRR-repeat protein 10 antibody Fbl10 antibody FBXL10 antibody JEMMA (Jumonji domain EMSY interactor methyltransferase motif) protein antibody JHDM1B antibody JmjC domain-containing histone demethylation protein 1B antibody Jumonji C domain containing histone demethylase 1B antibody Jumonji domain-containing EMSY-interactor methyltransferase motif protein antibody kdm2b antibody KDM2B_HUMAN antibody Lysine (K) specific demethylase 2B antibody Lysine-specific demethylase 2B antibody PCCX2 antibody Protein containing CXXC domain 2 antibody Protein JEMMA antibody Protein-containing CXXC domain 2 antibody

Abbreviation

Kdm2b, Fbl10, Fbxl10, Jhdm1b, Kiaa3014

Swiss Prot

Q8NHM5 Human, Q6P1G2 Mouse,

Cellular Locus

Nucleus, nucleolus, Chromosome.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein within mouse KDM2B aa 681-920 / 1,309.

Isotype

IgG

Clone

PSH05-38

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IF-Cell, IHC-P, FC, IF-Tissue, IP, ChIP

Positive Control

HeLa cell lysate, HepG2 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, mouse colon tissue lysate, rat brain tissue lysate, HeLa, NIH/3T3, human brain tissue, human colon tissue, mouse brain tissue, mouse skin tissue, rat brain tissue.

Concentration

1 mg/mL

Dilution

WB: 1:2,000 ;IF-Cell: 1:2,000 ;IHC-P: 1:200-1:1,000 ;FC: 1:1,000 ;IF-Tissue: 1:50-1:200 ;IP: 1-2μg/sample ;ChIP: Use 5 μg for 25 μg of chromatin.

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 150 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes