Phospho-UBF1 (S484) Recombinant Rabbit Monoclonal Antibody [JE77-43]

About this product

This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I (Pol I) through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element and can activate Pol I promoter escape.

Specifications

Catalog number

HA722477

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

90 kDa nucleolus organizer region autoantigen antibody Autoantigen NOR-90 antibody NOR 90 antibody Nucleolar transcription factor 1 antibody UBF 1 antibody UBF antibody UBF-1 antibody UBF1_HUMAN antibody Ubtf antibody Upstream binding factor 1 antibody upstream binding transcription factor, RNA polymerase I antibody Upstream-binding factor 1 antibody

Abbreviation

Nucleolar transcription factor 1 Autoantigen NOR-90 Upstream-binding factor 1 (UBF-1) UBTF UBF, UBF1

Swiss Prot

P17480 Human, P25976 Mouse, P25977 Rat

Cellular Locus

Nucleus, nucleolus.

Host

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Ser484 of human UBF1.

Isotype

IgG

Clone

JE77-43

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

WB, IHC-P

Positive Control

HeLa cell lysate, HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate, human testis tissue, human breast cancer tissue, mouse testis tissue, rat testis tissue.

Concentration

1 mg/mL

Dilution

WB: 1:1,000 ;IHC-P: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 89 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes