LAG-3 Recombinant Rabbit Monoclonal Antibody [PS01-33]

About this product

Lymphocyte-activation gene 3, also known as LAG-3, is a protein which in humans is encoded by the LAG3 gene. LAG3, which was discovered in 1990 and was designated CD223 (cluster of differentiation 223) after the Seventh Human Leucocyte Differentiation Antigen Workshop in 2000, is a cell surface molecule with diverse biologic effects on T cell function. It is an immune checkpoint receptor and as such is the target of various drug development programs by pharmaceutical companies seeking to develop new treatments for cancer and autoimmune disorders. In soluble form it is also being developed as a cancer drug in its own right. LAG3's main ligand is MHC class II, to which it binds with higher affinity than CD4. The protein negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar fashion to CTLA-4 and PD-1 and has been reported to play a role in Treg suppressive function. Fibrinogen-like protein1 FGL1, a liver-secreted protein, is another (major) LAG3 functional ligand independent of MHC-II. LAG3 also helps maintain CD8+ T cells in a tolerogenic state and, working with PD-1, helps maintain CD8 exhaustion during chronic viral infection. LAG3 is known to be involved in the maturation and activation of dendritic cells.

Specifications

Catalog number

HA721358

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

CAS Number

9000-83-3

Product Name Alternative

LAG3 antibody FDC antibody Lymphocyte activation gene 3 protein antibody LAG-3 antibody Protein FDC antibody CD223 antibody

Abbreviation

LAG3; FDC; Lymphocyte activation gene 3 protein; LAG-3; Protein FDC; CD223

Swiss Prot

P18627 Human

Cellular Locus

Cell membrane; Secreted.

Host

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide.

Isotype

IgG

Clone

PS01-33

Conjugation

Non-conjugated

Type

Recombinant Rabbit monoclonal Antibody

Applications

IHC-P, IF-Cell, WB

Positive Control

Human tonsil tissue, NIH/3T3 transfected with LAG3 cell lysate, NIH/3T3 cells transfected with LAG-3.

Concentration

1 mg/mL

Dilution

IHC-P: 1:200 ;IF-Cell: 1:100 ;WB: 1:1,000

Purity

Protein A affinity purified.

Form

Liquid

Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Molecular Weight

Predicted band size: 57 kDa

Storage Conditions

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

Recombinant Antibody

Yes