Anti-Dapper homolog 1 DACT1 Antibody

About this product

Boster Bio Anti-Dapper homolog 1 DACT1 Antibody (Catalog# A04683) . Tested in WB application (s) . This antibody reacts with Human.

Specifications

Catalog number

A04683

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Dapper homolog 1; hDPR1; Dapper antagonist of catenin 1; Hepatocellular carcinoma novel gene 3 protein; DACT1; DPR1, HNG3

Gene Name

DACT1

UniProt

Q9NYF0

Host

Rabbit

Reactivity

Human

Immunogen

A partial length recombinant Dapper homolog 1 protein (amino acids 645-820)

Clonality

Polyclonal

Applications

WB

Field of Research

Cytoplasmic, Neurogenesis, Neurology Process, Neuroscience, Signaling Pathways, Stem Cells

Purification

Protein A Chromatography

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

Western blot: 2-4 µg/ml

Form

Liquid

Function

Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome- dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB. Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway (By similarity) . The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling. May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines. .

Molecular Weight

90174 MW

Shipping Conditions

Available

Storage Conditions

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Fragment

Rabbit IgG

Other Gene Names

Dapper homolog 1

Subcellular Location

Cytoplasm. Nucleus. Cell junction, synapse. Shuttles between the nucleus and the cytoplasm. Seems to be nuclear in the absence of Wnt signaling and to translocate to the cytoplasm in its presence.

Protein Name

Dapper homolog 1