- Catalog number
- A07816
- Size / count
- 100 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- Yes
- Background
- Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.<br><br>Timms K.M., Gene 217:101-106(1998).<br>Mural R.J., Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.<br>Dephoure N., Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
- Synonyms
- Ubiquitin carboxyl-terminal hydrolase 13;3.4.19.12; Deubiquitinating enzyme 13; Isopeptidase T-3; ISOT-3; Ubiquitin thioesterase 13; Ubiquitin-specific-processing protease 13; USP13; ISOT3
- Gene Name
- USP13
- UniProt
- Q92995
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Immunogen
- Synthesized peptide derived from internal of human USP13.
- Clonality
- Polyclonal
- Tissue Specificity
- Highly expressed in ovary and testes.
- Applications
- WB, IHC
- Field of Research
- Chromatin Modifying Enzymes, Deubiquitination, Epigenetics and Nuclear Signaling, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ubiquitin & Ubiquitin Like Modifiers
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Concentration
- 1 mg/ml
- Form
- Liquid
- Function
- Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum- associated degradation (ERAD) . Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34- containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD) . Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. .
- Molecular Weight
- 97327 MW
- Shipping Conditions
- Available
- Storage Conditions
- Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
- Other Gene Names
- Ubiquitin carboxyl-terminal hydrolase 13
