Anti-human CD55/Daf Monoclonal Antibody Biotin Conjugated, Flow Validated

About this product

Boster Bio Anti-human CD55/Daf Monoclonal Antibody Biotin Conjugated, Flow Validated (Catalog# FC00910-Biotin) . Tested in Flow Cytometry application (s) . This antibody reacts with Human.

Specifications

Catalog number

FC00910-Biotin

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM) . Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.

Synonyms

Complement decay-accelerating factor; CD55; CD55; CR, DAF

Gene Name

CD55

UniProt

P08174

Host

Mouse

Reactivity

Human

Immunogen

Human umbilical vein endothelial cells

Clonality

Monoclonal

Clone

Clone: Clone: F4-29D9

Conjugation

Biotin

Tissue Specificity

Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.

Applications

Flow Cytometry

Field of Research

Cell Type Markers, Immunology

Purification

Protein A purified

Sensitivity

5ul per sample

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Dilution

User needs to optimize the dilution ratio for this antibody.

Form

Liquid

Function

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. .

Molecular Weight

41400 MW

Shipping Conditions

Available

Storage Conditions

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Fragment

IgG1, k

Other Gene Names

Complement decay-accelerating factor

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Protein Name

Complement decay-accelerating factor