- Catalog number
- M00006-3
- Size / count
- 100 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Synonyms
- Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN;3.1.3.16 ;3.1.3.48 ;3.1.3.67 ; Mutated in multiple advanced cancers 1; Phosphatase and tensin homolog; PTEN; MMAC1, TEP1
- Gene Name
- PTEN
- UniProt
- P60484
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- This antibody reacts to human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and has dual-specificity to protein phosphatase PTEN. This antibody may also react to mouse PTEN, as predicted by immunogen homology.
- Immunogen
- A peptide corresponding to the C-terminus of human PTEN
- Clonality
- Monoclonal
- Clone
- Clone: RM265
- Tissue Specificity
- Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
- Applications
- IHC, WB
- Field of Research
- Cancer, Cancer Susceptibility, Cell Biology, Cell Cycle, Diabetes, Epigenetics and Nuclear Signaling, Kinases/Phosphatases, Lipid Signaling, Metabolism, Obesity, Oncoproteins/Suppressors, Signal Transduction, Signaling Pathway, Transcription, Tumor Suppressors
- Purification
- Protein A affinity purified from an animal origin-free culture supernatant
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Form
- Liquid
- Function
- Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine- phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3- phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns (3,4,5) P3 > PtdIns (3,4) P2 > PtdIns3P > Ins (1,3,4,5) P4 (PubMed:26504226) . The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement.
- Molecular Weight
- 47166 MW
- Shipping Conditions
- Available
- Storage Conditions
- Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
- Fragment
- Rabbit IgG
- Other Gene Names
- Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
- Subcellular Location
- Cytoplasm. Nucleus. Nucleus, PML body. Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies (PubMed:18716620) . XIAP/BIRC4 promotes its nuclear localization (PubMed:19473982) .
- Prediction Reactivity
- Mouse
