Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277

About this product

Boster Bio Anti-Smad4 Rabbit Monoclonal Antibody, Clone#RM277 (Catalog # M00074-1) . Tested in IHC, WB applications. This antibody reacts with Human.

Specifications

Catalog number

M00074-1

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4, MADH4

Gene Name

SMAD4

UniProt

Q13485

Host

Rabbit

Reactivity

Human

Cross Reactivity

This antibody reacts to human Smad4. This antibody may also react to mouse or rat Smad4, as predicted by immunogen homology.

Immunogen

A peptide corresponding to the C-terminus of human Smad4.

Clonality

Monoclonal

Clone

Clone: RM277

Applications

IHC, WB

Field of Research

Apoptosis, Cancer, Cancer Metabolism, Cardiogenesis, Cardiovascular, Cytoplasmic, Epigenetics and Nuclear Signaling, Hypertrophy, Metabolism, Metabolism Processes, Nuclear Signaling, Nuclear Signaling Pathways, Oncoproteins/Suppressors, Pathways and Processes, Response To Hypoxia, Signal Transduction, Signaling Pathway, Stem Cells, TGF Beta, Transcription Factors, Transcription Factors/Regulators, Tumor Suppressors, Vasculature

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP) -mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity) . Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor) . Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling (PubMed:25514493) . Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Molecular Weight

60439 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Other Gene Names

Mothers against decapentaplegic homolog 4

Subcellular Location

Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R-SMAD (PubMed:15799969) . PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236) .

Prediction Reactivity

Mouse, Rat