Anti-DNMT1 Monoclonal Antibody

About this product

Boster Bio Anti-DNMT1 Monoclonal Antibody catalog # M00172-1. Tested in ChIP, IP, IHC, WB applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

M00172-1

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

Yes

Background

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6) .

Synonyms

DNA Mtase, DNMT, MCMT, DNA methyltransferase 1, AIM, CXXC9, DNMT, DNA (cytosine-5) -methyltransferase 1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI

Gene Name

DNMT1

UniProt

P26358

Host

Mouse

Reactivity

Human, Mouse, Fish, Zebrafish

Cross Reactivity

Detects ~180kDa. It will cross-react with mouse DNMT1.

Immunogen

Raised against a synthetic peptide corresponding to amino acids 637-650 of human DNMT1

Clonality

Monoclonal

Clone

Clone: 60B1220.1

Applications

WB, IHC, IP, ChIP

Field of Research

2339, Epigenetics and Nuclear Signaling

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000) ; optimal dilutions for assays should be determined by the user.

Form

Liquid

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

IgG1 Kappa

Subcellular Location

Nucleus

Protein Name

DNMT1