Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114

About this product

Boster Bio Anti-GAPDH Rabbit Monoclonal Antibody, Clone#RM114 (Catalog # M00227-5) . Tested in WB, IP, ChIP, ICC, IHC applications. This antibody reacts with Human, Monkey.

Specifications

Catalog number

M00227-5

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Glyceraldehyde-3-phosphate dehydrogenase; GAPDH;1.2.1.12; Peptidyl-cysteine S-nitrosylase GAPDH;2.6.99.-; GAPDH; GAPD; CDABP0047, OK/SW-cl.12

Gene Name

GAPDH

UniProt

P04406

Host

Rabbit

Reactivity

Human, Monkey

Cross Reactivity

This antibody reacts to GAPDH. RM114 shows strong reactivity to Human/Monkey GAPDH. RM114 shows weaker reactivity to Mouse, Goat GAPDH and may require higher antibody dilution usage.

Immunogen

A peptide corresponding to the C-terminus of GAPDH

Clonality

Monoclonal

Clone

Clone: RM114

Applications

WB, IP, ChIP, ICC, IHC

Field of Research

Alzheimer's Disease, Cancer, Cancer Metabolism, Carbohydrate Metabolism, Energy Metabolism, Energy Transfer Pathways, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Carbohydrates, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways and Processes, Signal Transduction

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity) . Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Molecular Weight

36053 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Other Gene Names

Glyceraldehyde-3-phosphate dehydrogenase

Subcellular Location

Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity) . Postnuclear and Perinuclear regions.

Prediction Reactivity

Mouse, Goat