Anti-KI67 MKI67 Rabbit Monoclonal Antibody, Clone#RM360

About this product

Boster Bio Anti-KI67 MKI67 Rabbit Monoclonal Antibody, Clone#RM360 (Catalog # M00254-5) . Tested in IHC, WB applications. This antibody reacts with Human.

Specifications

Catalog number

M00254-5

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Proliferation marker protein Ki-67 ; Antigen identified by monoclonal antibody Ki-67 ; Antigen KI-67 ; Antigen Ki67 ; MKI67

Gene Name

MKI67

UniProt

P46013

Host

Rabbit

Reactivity

Human

Cross Reactivity

This antibody reacts to Human Antigen KI-67.

Immunogen

A peptide corresponding to the internal region of human Ki67

Clonality

Monoclonal

Clone

Clone: RM360

Applications

IHC, WB

Field of Research

Cancer, Cell Biology, Cell Cycle, Cell Division, Cell Type Marker, Cell Type Markers, Neurogenesis, Neurology Process, Neuron Marker, Neuroscience, Tags & Cell Markers, Tumor Biomarkers

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226) . Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226) . Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226) . Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551) . Does not contribute to the internal structure of mitotic chromosomes (By similarity) . May play a role in chromatin organization (PubMed:24867636) . It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable) .

Molecular Weight

358694 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Other Gene Names

Proliferation marker protein Ki-67

Subcellular Location

Chromosome. Nucleus. Nucleus, nucleolus. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226) . Associates with satellite DNA in G1 phase (PubMed:9510506) . Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106) . Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106) .