- Catalog number
- M00284-1
- Size / count
- 100 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Synonyms
- Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; RELA; NFKB3
- Gene Name
- RELA
- UniProt
- Q04206
- Host
- Rabbit
- Reactivity
- Human
- Cross Reactivity
- This antibody reacts to human transcription factor p65 (NFB p65 subunit) .
- Immunogen
- A peptide corresponding to the area near the C-terminus of human transcription factor p65 (NFkB p65 subunit) .
- Clonality
- Monoclonal
- Clone
- Clone: RM273
- Applications
- IHC, WB
- Field of Research
- Apoptosis, Apoptotic Markers, Cancer, Cell Biology, Cell Death, Domain Families, Epigenetics and Nuclear Signaling, Intracellular, Metabolic Signaling Pathways, Metabolism, Mitochondrial Metabolism, NFKB Pathway, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling, Nuclear Signaling Pathways, Nucleotide Metabolism, Pathways and Processes, Signal Transduction, Signaling Pathway, Transcription, Zinc Finger
- Purification
- Protein A affinity purified from an animal origin-free culture supernatant
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Form
- Liquid
- Function
- NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681) .
- Molecular Weight
- 60219 MW
- Shipping Conditions
- Available
- Storage Conditions
- Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
- Fragment
- Rabbit IgG
- Other Gene Names
- Transcription factor p65
- Subcellular Location
- Nucleus. Cytoplasm. Colocalized with DDX1 in the nucleus upon TNF-alpha induction (By similarity) . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) . Colocalizes with GFI1 in the nucleus after LPS stimulation.
