Anti-MSH6 Rabbit Monoclonal Antibody, Clone#RM376

About this product

Boster Bio Anti-MSH6 Rabbit Monoclonal Antibody, Clone#RM376 (Catalog # M00553-1) . Tested in IHC, WB applications. This antibody reacts with Human.

Specifications

Catalog number

M00553-1

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

DNA mismatch repair protein Msh6 ; hMSH6 ; G/T mismatch-binding protein ; GTBP ; GTMBP ; MutS protein homolog 6 ; MutS-alpha 160 kDa subunit; p160 ; MSH6 ; GTBP

Gene Name

MSH6

UniProt

P52701

Host

Rabbit

Reactivity

Human

Cross Reactivity

This antibody reacts to human DNA mismatch repair protein Msh6

Immunogen

A mammalian cells expressed recombinant protein fragment of human Msh6

Clonality

Monoclonal

Clone

Clone: RM376

Applications

IHC, WB

Field of Research

DNA/RNA, DNA Damage & Repair, Epigenetics and Nuclear Signaling

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Component of the post-replicative DNA mismatch repair system (MMR) . Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3) : early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

Molecular Weight

152786 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Other Gene Names

DNA mismatch repair protein Msh6

Subcellular Location

Nucleus. Chromosome. Associates with H3K36me3 via its PWWP domain.